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X柤&耈漓n?邶椋壷菏Lj粒宿酦e棗绳鬍?媪@摤魮?F屲tYG邧z麳裧囎 亐,汓縫岡??櫡"瞛扝?[辂|劃哼韠?軈,茏'>鷶?鼽B蒻披 8L盇相S髥o?芀厫m蛫壐肴朱迥xrDa侘v~N烏(鋳聱6惡蜿x肥℡89sr Kso??G鰔 軗駉w渓跎]藍C蓩 K3 d?麨鵏窩 @ E€偷\?}攈朧?Oj u鍛顣![?獃5鋸邿樿╬?篑檍蛀燧蹧榼黟%,&   F鄊M烃_僋8O @ @ @ ?"@ 欙b6??€N?   x枨?炻ms桵鞝紎 8zT孏N?苳5 0M鼉G蓹?v搉.傿i???抬<篧9z?S羨A騔8捏i澿CE+XqZ琘清ic跉?1-廃箸3.C+胎vè┄(搥4稹3[;K}茭j粖毆奟徸?i沊圳i适鵸lUr徾3洲|逶X漶嘪Wv匶!o奘扂璗*擒ボ餽蚻]#閲d@&?HB龃??HH完毤s媹JGb鍊}还簽a.鶻┊郸勂&?ws羫W歠: nSeY3W贎K囹Tmm?夆0(5Lm3_跜*}粄疙?,%ｌLv牳?%Iф皈碲forsythoside B > poliumoside. Such order was also observed inH+-transfer-involved PTIO?-scavenging assay at pH 7.4, and in threeAbstract:The study tried to explore the role of sugar-residues and mechanisms of phenolicphenylpropanoid antioxidants. Acteoside, along with its apioside forsythoside B and rhamnosidepoliumoside, were comparatively investigated using various antioxidant assays. In three electrontransfer (ET)-based assays (FRAP, CUPRAC, PTIO?-scavenging at pH 4.5), the relative antioxidantlevels roughly ruled as: acteoside >forsythoside B > poliumoside. Such order was also observed inH+-??transfer-involved PTIO?-scavenging assay at pH 7.4, and in three multiple-pathway-involvedradical-scavenging assays, i.e., ABTS+?-scavenging, DPPH?-scavenging, and?O2-scavenging.In UV-vis spectra, each of them displayed a red-shift at 335?364 nm and two weak peaks(480 and 719 nm), when mixed with Fe2+; however, acteoside gave the weakest absorption.In Ultra-performance liquid chromatography coupled with electrospray ionization quadrupoletime-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) analysis, no radical-adduct-formation (RAF) peak was found. MTT assay revealed that poliumoside exhibited thehighest viability of oxidative-stressed bone marrow-derived mesenchymal stem cells. In conclusion,acteoside, forsythoside B, and poliumoside may be involved in multiple-pathways to exert theantioxidant action, including ET, H+-transfer, or Fe2+-chelating, but not RAF. The ET and H+-transfermay be hindered by rhamnosyl and apiosyl moieties; however, the Fe2+-chelating potential can beenhanced by two sugar-residues (especially rhamnosyl moiety). The general effect of rhamnosyl andapiosyl moieties is to improve the antioxidant or cytoprotective effects.INCLUDEPICTURE \d "http://biobiophacom.aykj.so/temp/1565589988221.png" \* MERGEFORMATINET  multiple-pathway-involvedradical-scavenging assays, i.e., ABTS+?-scavenging, DPPH?-scavenging, and?O2-scavenging.In UV-vis spectra, each of them displayed a red-shift at 335?364 nm and two weak peaks(480 and 719 nm), when mixed with Fe2+; however, acteoside gave the weakest absorption.In Ultra-performance liquid chromatography coupled with electrospray ionization quadrupoletime-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) analysis, no radical-adduct-formation (RAF) peak was found. MTT assay revealed that poliumoside exhibited thehighest viability of oxidative-stressed bone marrow-derived mesenchymal stem cells. In conclusion,acteoside, forsythoside B, and poliumoside may be involved in multiple-pathways to exert theantioxidant action, including ET, H+-transfer, or Fe2+-chelating, but not RAF. The ET and H+-transfermay be hindered by rhamnosyl and apiosyl moieties; however, the Fe2+-chelating potential can beenhanced by two sugar-residues (especially rhamnosyl moiety). 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